Guidelines for using Phire Hot Start DNA Polymerase
Phire Hot Start DNA Polymerase is provided with 5x Phire Reaction Buffer. The buffer contains 1.5 mM MgCl2 at final reaction concentration. A separate tube of DMSO is provided for further optimization.
Basic reaction conditions for PCR
Carefully mix and centrifuge all tubes before opening to improve recovery. When using Phire Hot Start DNA Polymerase, the PCR setup can be performed at room temperature. Prepare a master mix for the appropriate number of samples. The DNA polymerase should be pipetted carefully and gently as the high glycerol content (50 %) in the storage buffer may otherwise lead to pipetting errors.