Media Recipes for Phusion Site-Directed Mutagenesis Kit (F-541)

Introduction

LB agar with antibiotics (per liter)
Tryptone 10 g
Yeast Extract 5 g
NaCl 10 g
Agar 15 g
Adjust pH to 7.0 with NaOH
Autoclave
Cool to 55°C and add the appropriate antibiotic:
e.g. ampicillin (final concentration 100 µg/ml) before pouring the plates.
For plates with blue-white color screening, add 80 µg/ml X-Gal and 1 mM IPTG.

SOC medium (per liter)
Tryptone 20 g
Yeast Extract 5 g
NaCl 0.5 g
KCl 0.186 g
Adjust pH to 7.0 with NaOH
Autoclave
Before use add sterile solutions:
1 M MgCl2 10 ml
1 M MgSO4 10 ml
1 M Glucose 20 ml