Suggested protocol for loading a sample (PCR Marker)


The following protocol is recommended for a 5 mm wide lane.


  1. Prepare lodaing mixture:
    Distilled water - 4 μl
    6X Blue Loading Dye - 1 μl
    DNA Ladder - 1 μl
    Total volume - 6 μl
  2. Mix gently
  3. Load onto the agarose gel
    Note: The components of the mixture should be scaled up or down, depending on the width of the agarose gel.