Carefully mix and centrifuge all tubes before opening to improve recovery. When using Phusion Hot Start DNA Polymerase, it is not necessary to perform the PCR setup on ice. Prepare a master mix for the appropriate number of samples to be amplified. The DNA polymerase should be pipetted carefully and gently as the high glycerol content (50%) in the storage buffer may otherwise lead to pipetting errors.
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The NEB PCR Cloning Kit allows direct cloning from any amplification reaction, with no purification needed!
The Gibson Assembly™ Cloning Kit is a robust method for assembling 1-2 DNA fragments, with a cloning vector, and ensuring high transformation efficiencies - all in a little less than 2 hours.