Control Peptide PB1 Protocol 1: Western Blotting

Protocol

1. Dissolve your peptide in water to a concentration of 1 mM.
2. Thaw the carrier protein at room temperature.
3. Mix the following (in order):
4. Incubate at room temperature for 15-60 minutes or at 4°C overnight.
5. Add 12.5 µl of 3X SDS Sample Buffer.
6. Heat at 95°C for 5 minutes.
7. Load 5-10 µl/lane (~ 25-50 ng) for Western blotting.