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  • Wash Off Unbound DNA (E2600)

    Protocol

    1. After incubating the DNA and MBD2-Fc/ Protein A Magnetic Beads, place the tube on the magnetic rack for 2–5 minutes to concentrate the beads on the inner wall of the tube.
    2. Carefully remove the supernatant with a pipette without disturbing the beads, and save it in a clean microcentrifuge tube. This saved supernatant is the non-captured DNA fraction. Store this sample on ice or at -20°C.
    3. Add 1000 μl of 1X Bind/Wash Buffer to the tube to remove of residual non-captured DNA.
    4. Mix the beads on a rotating mixer for 3 minutes at room temperature.
    5. Place the tube on the magnetic rack for 2–5 minutes to concentrate the beads on the inner wall of the tube. Remove the supernatant.
    6. Repeat steps 3–5 two more times.