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- Prepare a 20 μl reaction as follows:
||1 pmol of DNA ends*
|Antarctic Phosphatase Rxn Buffer (10X)
|| 5 units
||to 20 μl**
- Incubate at 37°C for 30 minutes.
- Stop reaction by heat-inactivation at 80°C for 2 minutes.
* Note: 1 pmol of DNA ends is about 1 μg of a 3 kb plasmid.
** Scale larger reaction volumes proportionally.