Suggested Loading a Sample (N0472)

Introduction

The following protocol is recommended for a 5 mm wide lane.

Protocol

  1. Prepare loading mixture:
    Distilled water: 4 µl
    6X Blue Loading Dye: 1 µl
    DNA Ladder: 1 µl   
    Total volume: 6 µl
  2. Mix gently
  3. Load onto the agarose gel 

Note: The components of the mixture should be scaled up or down, depending on the width of the agarose gel.