• My NEB
  • Print
  • PDF
  • Suggested Protocol for Loading a Sample (P7702)

    Protocol

    1. Mix Protein Marker. Bring the desired amount of the Protein Marker (7 µl for mini-gels and 15 µl for full length gels) over to a separate tube.
    2. Heat the Marker to 95-100°C for 3-5 minutes.
    3. After a quick microcentrifuge spin, load directly on to a gel. To ensure uniform mobility, load an equal volume of 1X Reducing SDS Loading Buffer into any unused wells.