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  • Suggested protocol for loading a sample (N3232)

    Overview

    The following protocol is recommended for a 5 mm wide lane.

    Protocol

    1. Prepare loading mixture: 
      Distilled water - 4 μl
      6X Blue Loading Dye - 1 μl
      DNA Ladder - 1 μl
      Total volume - 6 μl
    2. Mix gently
    3. Load onto the agarose gel
      Note: The components of the mixture should be scaled up or down, depending on the width of the agarose gel.