• My NEB
  • Print
  • PDF
  • Suggested protocol for loading a sample

    For use with λ DNA-BstEII Digest (N3014) and λ DNA- HindIII Digest (N3012)


    The following protocol is recommended for a 5 mm wide lane.


    1. Prepare loading mixture:
      Distilled water:               3 μl
      6X Blue Loading Dye:      1 μl
      DNA Ladder:                  2 μl
      Total volume:                 6 μl
    2. Mix gently
    3. Load onto the agarose gel
      Note: The components of the mixture should be scaled up or down, depending on the width of the agarose gel.