Suggested protocol for loading a sample

For use with λ DNA-BstEII Digest (N3014) and λ DNA- HindIII Digest (N3012)

Overview

The following protocol is recommended for a 5 mm wide lane.

Protocol

  1. Prepare loading mixture:
    Distilled water:               3 μl
    6X Blue Loading Dye:      1 μl
    DNA Ladder:                  2 μl
    Total volume:                 6 μl
  2. Mix gently
  3. Load onto the agarose gel
    Note: The components of the mixture should be scaled up or down, depending on the width of the agarose gel.