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  • Standard E. coli DNA Gyrase Reaction (M0306)

    Overview

    Mix the following components in an eppendorf tube as follows:

    5X DNA Gyrase reaction buffer: 6ul
    DNA Gyrase (M0306): 1ul
    DNA (relax, circular): 500ng-2.5ug
    H2O: to a total of 30ul
    Incubate the mixture for 30min @37°C