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  • Sample preparation using provided buffer and denaturing conditions (N0364)

    Overview

    This method utilizes the 2X ssRNA Ladder Loading Buffer provided and samples should be run on a denaturing polyacrylamide-urea gel prepared with 1X TBE.

    Protocol

    1. Combine on ice:
      Low Range ssRNA Ladder (500 µg/ml):   0.5 µl (0.25 µg)
      H2O (RNase-free):   4.5 µl
      2X ssRNA Ladder Loading Buffer:   5 µl
      Total Volume:   10 µl
    2. Heat at 65°C for 5 minutes, chill on ice, load entire sample on gel.