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  • Reverse Transcription (E6160)

    Protocol

    1. Mix the following components in a sterile nuclease-free tube:
      3´ 5´ Ligated RNA from step 12: 11 μl
      Deoxynucleotide Solution Mix: 1 μl
      5X First Strand Buffer (Supplied with SuperScript® II): 4 μl
      0.1 M DTT (Supplied with SuperScript® II): 2 μl
      Murine RNase Inhibitor: 1 μl 
      ------------------------------------------------------------
      Total volume: 19 μl
    2. Heat the sample for 2 minutes at 42°C.
    3. Add 1 μl SuperScript® II Reverse Transcriptase.
    4. Incubate for 1 hour at 42°C.