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  • Removal of Single-Stranded Extension

    Protocol

    1. Suspend DNA (0.1 μg/μl) in 1X Mung Bean Nuclease Buffer or 1X NEBuffer 1, 2 or 4.
    2. Add 1.0 unit of Mung Bean Nuclease per μg DNA.
    3. Incubate at 30°C for 30 minutes.
    4. Inactivate the enzyme by phenol/chloroform extraction or by addition of SDS to 0.01%.
    5. Recover the DNA by ethanol precipitation.