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  • Purification of labeled probe generate form the NEBlot Kit

    Overview

    Purification and Use of Labeled Probes

    Probes synthesized can be separated from unincorporated nucleotides by gel filtration on Sephadex® G-50, Spin Column Elutips® or similar size exclusion media.  Following purification, labeled DNA should be prepared for hybridization as follows.

    Protocol

    1. Denature by heating in boiling H2O bath 95-100oC for 5 minutes.
    2. Quickly place in ice bath for 5 minutes.  DNA may be used directly in hybridization experiment, or store at -20oC.