Purification and Use of Labeled Probes
Probes synthesized can be separated from unincorporated nucleotides by gel filtration on Sephadex® G-50, Spin Column Elutips® or similar size exclusion media. Following purification, labeled DNA should be prepared for hybridization as follows.
- Denature by heating in boiling H2O bath 95-100oC for 5 minutes.
- Quickly place in ice bath for 5 minutes. DNA may be used directly in hybridization experiment, or store at -20oC.