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  • PURExpress Disulfide Bond Enhancer (E6820)

    Protocol

    1. Please refer to the PURExpress In Vitro Protein Synthesis Kit manual (NEB #E6800) for details about PURExpress reactions.
    2. During set-up of PURExpress reactions, add 1 µl of PURExpress Disulfide Bond Enhancer 1 and 1 µl of PURExpress Disulfide Bond Enhancer 2 per 25 µl of PURExpress reaction. If reactions larger or smaller than 25 µl are to be performed, the amount of the PURExpress Disulfide Bond Enhancer added should be scaled accordingly.
    3. Allow reaction to proceed for at least 2 hrs at desired temperature (usually 37°C).
    4. Place reactions on ice if they will be analyzed within 3–4 hrs; otherwise store at -20°C until needed.