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  • Protocol II: 1 M Tris-HCl Buffer Stock Solution (1 liter)

    Protocol

    1. Solution A: 
      Dissolve 121.14 g Tris (American Bioanalytical #AB14042) in 800 ml dH2O.
    2. Adjust pH to 7.0 with the appropriate volume of concentrated HCl. Bring final volume to 1 liter with deionized water.
    3. Autoclave and store at room temperature.