Protocol for Oligonucleotide Adenylation (E2610)
- Set up the following reaction in a sterile microfuge tube:
Components Volume Phosphorylated DNA
100 pmol (5 pmol/µl) 10X 5´ DNA Adenylation
2 µl 1 mM ATP 2 µl Mth RNA Ligase 2 µl (100 pmol) Nuclease-free Water to 20 µl
- Incubate at 65°C for 1 hour.
- Inactivate the enzyme by incubation at 85°C for 5 minutes.