Genomic DNA Digestion using LpnPI (R0663)

Protocol

  1. Set up the following reaction in a sterile microcentrifuge tube (it is important to add LpnPI last):
    DNA (0.5 to 1 μg) 1–5 μl
    10X NEBuffer 4 3 μl
    BSA 0.3 μl
    30X Enzyme Activator Solution 1 μl
    LpnPI 0.5–1 μl ( 2 to 4 units)
    Nuclease-free water to 30 μl
  2. Incubate at 37°C for 4-8 hours.