Genomic DNA Digestion using FspEI (R0662)

Protocol

  1. Set up the following reaction in a sterile microcentrifuge tube (it is important to add FspEl last):
    DNA (0.5 to 1 μg) 1–5 μl
    10X CutSmart Buffer
    3 μl
    30X Enzyme Activator Solution 1 μl
    FspEl 0.5–1 μl ( 2.5 to 5 units)
    Nuclease-free water to 30 μl
  2. Incubate at 37°C for 4–8 hours.

   Note: Selling concentration is 5,000 U/ml this used to be 4,000 U/ml.