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  • Genomic DNA Digestion using FspEI (R0662)

    Protocol

    1. Set up the following reaction in a sterile microcentrifuge tube (it is important to add FspEl last):
      DNA (0.5 to 1 μg) 1–5 μl
      10X NEBuffer 4 3 μl
      BSA 0.3 μl
      30X Enzyme Activator Solution 1 μl
      FspEl 0.5–1 μl ( 2 to 4 units)
      Nuclease-free water to 30 μl
    2. Incubate at 37°C for 4–8 hours.