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  • Denaturing PAGE/Urea or Denaturing Agarose Gel (B0363)

    Protocol

    1. Add sample to an equal volume of RNA Loading Dye, (2X). Mix well.
    2. Heat at 65–70°C for 5–10 minutes to denature RNA.
    3. While heating the samples, setup the gel box and flush urea out of the wells with running buffer using a large tip.
    4. Load samples.

      For Non-denaturing PAGE/Agarose Gel:
      Use protocol described above, but do not heat the samples.