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  • A Typical Tailing Reaction (M0337)

    1. Combine the following in a sterile microcentrifuge tube:
      10X NEBuffer   2.5 µl 
       UTP   0.5 mM final
       RNA  >100 pmol
       RNase Inhibitor* (40 units/µl)  1 µl
       Poly(U) Polymerase (2 units/µl)  1 µl
       H2O  to 25µl
    2. Incubate at 37°C for 10 minutes. 

      * RNase Inhibitor is recommended but not required.