A Typical Tailing Reaction (M0337)

  1. Combine the following in a sterile microcentrifuge tube:
    10X NEBuffer   2.5 µl 
     UTP   0.5 mM final
     RNA  >100 pmol
     RNase Inhibitor* (40 units/µl)  1 µl
     Poly(U) Polymerase (2 units/µl)  1 µl
     H2O  to 25µl
  2. Incubate at 37°C for 10 minutes. 

    * RNase Inhibitor is recommended but not required.