Protocol for a Typical miRNA Methylation Reaction (M0228)

Combine the following components in sterile microfuge tube:

10X NEBuffer 2.5 µl
3.2 mM SAM 1 µl
miRNA duplex (100–500 ng X
H2O X
RNase inhibitor (optional) 20 units
HEN 1 RNA Methyltransferase (50 units/µl) 1 μl
Total reaction volume 25 μl
Incubate at 37°C for 1 hour.