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  • Protocol for a Typical miRNA Methylation Reaction (M0228)

    Combine the following components in sterile microfuge tube:

    10X NEBuffer 2.5 µl
    3.2 mM SAM 1 µl
    miRNA duplex (100–500 ng X
    H2O X
    RNase inhibitor (optional) 20 units
    HEN 1 RNA Methyltransferase (50 units/µl) 1 μl
    Total reaction volume 25 μl
    Incubate at 37°C for 1 hour.