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  • NEBNext Quick Ligation Module Protocol (E6070)

    Protocol

    1. Mix the following components in a sterile microfuge tube: 
      End Repaired, Blunt or dA-Tailed DNA:  30 μl
      Quick Ligation Reaction Buffer (5X):  10 μl
      DNA Adaptors(not provided please use adaptors appropriate to specific application):  5 μl
      Quick T4 DNA Ligase:  5 μl 
      -------------------------------------------------------------
      Total volume:  50 μl
    2. Incubate in a thermal cycler for 15 minutes at 20°C.
    3. Purify DNA sample on one column and elute in 25 μl of sterile dH2O or elution buffer.