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  • NEBNext Quick Ligation Module Protocol (E6056)


    1. Mix the following components in a sterile microfuge tube:

      End Repaired, Blunt or dA-Tailed DNA:   variable
      NEBNext Quick Ligation Reaction Buffer (5X):   10 μl
      10 µM DNA Adaptors (not provided please use adaptors appropriate to specific application):   variable
      Quick T4 DNA Ligase:   5 μl
      Sterile H2O:   variable
      Total volume:   50 μl
    2. Incubate in a thermal cycler for 15 minutes at 20°C.
    3. Purify DNA sample on one column.