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  • NEBNext End Repair Module Protocol (E6260)


    Starting Material: 10 ng of chromatin-immunoprecipitated (ChIP) qPCR verified or control DNA in ≤ 85 μl of water or elution buffer.


    1. Mix the following components in a sterile microfuge tube:
      ChIP DNA 1–85 μl
      NEBNext End Repair Reaction Buffer (10X) 10 μl
      NEBNext End Repair Enzyme Mix 5 μl
      Sterile H2O variable
      Total volume 100 μl
    2. Incubate for 30 minutes at room temperature.
    3. Purify the end repaired DNA using Agencourt AMPure XP beads.