NEBNext DNA Sample Prep Master Mix Set for 454 End Repair Module Protocol (E6070)


Starting Material: 1–5 μg of DNA Fragmented to 100–1000 bp in ≤ 85 μl


  1. Mix the following components in a sterile microfuge tube: 
    Fragmented DNA:  1–85 μl
    NEBNext End Repair Reaction Buffer (10X):  10 μl
    NEBNext End Repair Enzyme Mix:  5 μl
    Sterile H2O for a final volume of 100 μl:  variable 
    Total volume:  100 μl
  2. Incubate in a thermal cycler for 30 minutes at 20°C.
  3. Purify DNA sample on one column and elute in 30 μl of sterile dH2O or elution buffer.