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  • NEBNext End Repair Module Protocol (E6060)

    Protocol

    1. Mix the following components in a sterile microfuge tube: 
      Fragmented DNA:   1–100 μl |
      NEBNext End Repair Reaction Buffer (10X):   20 μl 
      NEBNext End Repair Enzyme Mix:   10 μl 
      Sterile H2O for a final volume of 100 μl:   variable 
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      Total volume:   200 μl
    2. Incubate in a thermal cycler for 30 minutes at 20°C.
    3. Purify DNA sample on one column.