NEBNext End Repair Module Protocol (E6050)

Introduction

Starting Material: 1-5 μg of DNA Fragmented to 100-1000 bp in ≤ 85 μl

Protocol

  1. Mix the following components in a sterile microfuge tube:
    Fragmented DNA:   variable
    NEBNext End Repair Reaction Buffer (10X):   10 μl
    NEBNext End Repair Enzyme Mix:   5 μl
    Sterile H2O for a final volume of 100 μl:   variable 
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    Total volume:   100 μl
  2. Incubate in a thermal cycler for 30 minutes at 20°C.
  3. Purify DNA sample on one column.