• My NEB
  • Print
  • PDF
  • NEBNext End Repair and dA-Tailing (E6115)


    1. In a 1.7 ml micro-centrifuge tube add:
      NEBuffer 2 (10X): 2.5 μl
      ATP: 2.5 μl
      dNTP Mix: 1.0 μl
      T4 DNA Polymerase: 1.0 μl
      PNK: 1.0 μl
      Taq DNA Polymerase: 1.0 μl
      Total Volume: 9.0 μl
    2. Mix by pipetting and add to the 16 μl purified, double stranded cDNA.
    3. Vortex briefly to mix, followed by a quick spin to collect all liquid from the sides of the tube.
    4. In a thermocycler, with the heated lid on, run the following program:
      20 minutes at 25°C
      20 minutes at 72°C
      Hold at 4°C