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  • NEBNext End Repair and dA-Tailing (E6090)

    Protocol

    1. Starting Material: 0.5 μg of DNA Fragmented to 100–1000 bp in 16 μl of TE.

      In a 1.7 ml micro-centrifuge tube add:
      End Prep Enzyme Mix 1.0 μl
      End Repair Reaction Buffer (10X) 2.5 μl
      Nuclease -free Water 5.5 μl
      Total 9.0 μl


    2. Mix by pipetting and add to the 16 μl fragmented DNA sample.

    3. Vortex briefly to mix, followed by a quick spin to collect all liquid from the sides of the tube.

    4. In a thermocycler, with the heated lid on, run the following program: 
      20 minutes @ 25°C 
      20 minutes @ 72°C 
      Hold at 4°C