NEBNext dA-Tailing Module Protocol (E6053)

Introduction

Starting Material: 1-5 μg of of end repaired, blunt DNA (100-1000 bp).

Protocol

  1. Mix the following components in a sterile microfuge tube:

    End Repaired, Blunt DNA:   variable
    NEBNext dA-Tailing Reaction Buffer (10X):   5 μl
    Klenow Fragment (3´→ 5´ exo):   3 μl
    Sterile H2O:   variable
    -------------------------------------------------------------
    Total volume:   50 μl
  2. Incubate in a thermal cycler for 30 minutes at 37°C.
  3. Purify DNA sample on one spin column.