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  • NEBNext dA-Tailing Module Protocol (E6053)

    Introduction

    Starting Material: 1-5 μg of of end repaired, blunt DNA (100-1000 bp).

    Protocol

    1. Mix the following components in a sterile microfuge tube:

      End Repaired, Blunt DNA:   variable
      NEBNext dA-Tailing Reaction Buffer (10X):   5 μl
      Klenow Fragment (3´→ 5´ exo):   3 μl
      Sterile H2O:   variable
      -------------------------------------------------------------
      Total volume:   50 μl
    2. Incubate in a thermal cycler for 30 minutes at 37°C.
    3. Purify DNA sample on one spin column.