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  • NEBNext Adaptor Ligation (E6080)

    Protocol

    1. After completion of the End Repair/dA-Tailing program add 1.0 μl of adaptor (or vector if generating an expression library), to the reaction tube. (Adaptors/vectors are not provided. Please use adaptors/vectors appropriate to specific application).

    2. Add 1.0 μl of Quick T4 DNA Ligase. Vortex briefly to mix, followed by a quick spin.

    3. Incubate for 10 minutes at 25°C.