1. Mix protein marker. Bring the desired amount of the Prestained Protein Marker over to a separate tube. For blotting: use 6 µl for mini-gels and 12 µl for full length gels. For visualizing during electrophoresis: use 15 µl for mini-gels and 30 µl for full length gels.
2. Heat the marker to 95-100°C for 3-5 minutes. If the marker has been already boiled upon receipt, don't heat again, directly go to Step 3.
3. After a quick microcentrifuge spin, load directly on to a gel. To ensure uniform mobility, load an equal volume of 1X reducing SDS Loading Buffer into any unused wells.