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  • Guidelines for ligation of single-stranded pre-adenlyated DNA adapters to the 3’-ends of single-stranded RNA

    Protocol

    1. Mix the following reagents:
      10 pmoles ssRNA
      20 pmoles pre-adenylated DNA adapter (e.g. NEB #S1315)
      1x T4 RNA Ligase 2 truncated K227Q reaction buffer
      200 U T4 RNA Ligase 2 truncated K227Q
      20 μl total volume
    2. Incubate reactions at 16°C overnight.