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  • Clean Up of Amplified Library (E6285)

    Protocol

    1. Add 100 µl (1X volume) of AMPure XP Beads to the sample and mix by pipetting up and down.

    2. Incubate for 5 minutes at room temperature.

    3. Pulse spin the tube and place in a magnetic rack for 2–3 minutes until the beads have collected to the side of the tube and the solution is clear.

    4. Carefully remove and discard the supernatant without disturbing the beads.

    5. Keep the tube on the magnet and add 500 μl freshly prepared 80% ethanol.

    6. Incubate at room temperature for 30 seconds and carefully remove and discard the supernatent.

    7. Repeat steps 5-6.

    8. Keeping the tube in the magnetic rack, with the cap open, air dry the beads for 5 minutes.

    9. Resuspend the beads in 25 µl of 0.1X TE. Mix well on a vortexer or by pipetting up and down, and put the tube on the magnetic rack until the solution is clear.

    10. Transfer approximately 20 µl to a clean tube.

    11. Assess library quality on the Bioanalyzer.