Capture Methylated CpG DNA (E2600)

Protocol

  1. Add 20 μl of 5X Bind/Wash Buffer to a clean 1.7 ml DNase-free microcentrifuge tube.
  2. Add 5 ng-10 μg of fragmented sample DNA, up to a total volume of 70 μl.
  3. Add 10 μl of well-mixed MBD2-Fc / Protein A Magnetic Beads.
  4. Bring the total volume of the reaction to 100 μl with DNase-free water.
  5. Incubate the reaction for 20 minutes at room temperature with rotation.