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  • Adaptor or Vector Ligation of cDNA Library (E6114)

    Protocol

    1. Mix the following components in a sterile microfuge tube: 

      End Repaired, Blunt DNA:   variable
      NEBNext Quick Ligation Reaction Buffer (5X):   40 μl
      DNA Adaptors or Vector variable T4 DNA Ligase:   10 μl
      Nuclease-Free Water:   variable 
      ------------------------------------------------------------------
      total volume:   200 μl
    2. Incubate in a thermal cycler for 15 minutes at 20°C.
    3. Purify DNA sample on one column.