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  • Adaptor Ligation of End Repaired DNA (E6260)

    Protocol

    1. Mix the following components in a sterile microfuge tube:
      End Repaired, Blunt DNA 30 μl
      NEBNext Quick Ligation Reaction Buffer (5X) 20 μl
      *P1 Adaptor (2.5 pmol / μl) 1 μl
      *P2 Adaptor (2.5 pmol / μl) 1 μl
      Quick T4 DNA Ligase 1 μl
      Sterile H2O 47 μl
      Total volume 100 μl
      *Adaptors are not included, use adaptors appropriate to specific application.
    2. Incubate at room temperature for 10 minutes.