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  • A Typical Exonuclease V Reaction (M0345)

    Introduction

    x μl sample DNA (~ 1 μg)
    3 μl NEBuffer4 (10X)
    3 μl 10 mM ATP
    y μl H20 (up to final volume of 30 μl)
    1 μl Exonuclease V (10 units)

    Protocol

    1. Incubate at 37°C for 30 minutes.
    2. To stop reaction add EDTA to 11 mM.
    3. Heat Inactivation 70°C for 30 minutes.
    4. Clean-up treated samples by column purification and/or ethanol precipitation.

      Note: Estimate amount of DNA to be removed by agarose gel electrophoresis or OD260. If > 1 μg scale up all reaction components proportionately.