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  • A Typical DNase I Reaction

    Protocol

    1. Resuspend 10 µg RNA in 1X DNase I Reaction Buffer to a final volume of 100 µl.
    2. Add 2 units of DNase I, mix thoroughly and incubate at 37°C for 10 minutes.
    3. Add 1 µl of 0.5 M EDTA (to a final concentration of 5 mM).
    4. Heat inactivate at 75°C for 10 minutes.