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  • A Typical Deadenylation Reaction (M0331)

    Combine the following components in sterile microfuge tube:

    10X NEBuffer 1 2 μl
    5–50 pmol 5´-adenylated DNA or RNA variable
    H2O variable
    5´ Deadenylase (50 units/μl) 1 μl
    Total reaction 20 μl

    Incubate at 30°C for 0.5–1 hour