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  • A Typical Deadenylation Reaction (M0331)

    Combine the following components in sterile microfuge tube:

    10X NEBuffer 2                                                2 μl
    5–50 pmol 5´-adenylated DNA or RNA         variable
    H2O                                                          variable
    5´ Deadenylase (20 units/μl)                            1 μl 
    -----------------------------------------------------------------------------
    Total reaction volume                                     20 μl
    Incubate at 30°C for 0.5–1 hour