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  • 5 Minute Transformation Protocol (C3022)


    A shortened transformation protocol resulting in approximately 10% efficiency compared to the standard protocol may be suitable for applications where a reduced total number of transformants is acceptable.

    Follow the High Efficiency Transformation Protocol above with the following changes:
    1. Steps 3 and 5 are reduced to 2 minutes.
    2. Omit outgrowth (step 7) completely for ampicillin-resistant plasmids or reduce the outgrowth time for other selective media as appropriate.