A shortened transformation protocol resulting in only 10% efficiency compared to the standard protocol may be suitable for applications where a reduced total number of transformants is acceptable.
Follow the transformation protocol with the following changes:
1. Steps 3 and 5 are reduced to 2 minutes.
2. Omit outgrowth (Step 7) completely for ampicillin-resistant plasmids or reduce the outgrowth time for other selective media as appropriate.