5 Minute Transformation Protocol (C2530)

Introduction

A shortened transformation protocol resulting in approximately 10% efficiency compared to the standard protocol may be suitable for applications where a reduced total number of transformants is acceptable.

Follow the Transformation Protocol with the following changes:

Protocol

  1. Steps 3 and 5 are reduced to 2 minutes.
  2. Omit outgrowth (step 7) completely for ampicillin-resistant plasmids or reduce the outgrowth time for other selective media as appropriate.