Suggested Protocol for Loading a Sample (P7702)
- Mix Protein Marker. Bring the desired amount of the Protein Marker (7 µl for mini-gels and 15 µl for full length gels) over to a separate tube.
- Heat the Marker to 95-100°C for 3-5 minutes.
- After a quick microcentrifuge spin, load directly on to a gel. To ensure uniform mobility, load an equal volume of 1X Reducing SDS Loading Buffer into any unused wells.