Suggested protocol for loading a DNA Ladder/marker
Protocols.io also provides an interactive version of this protocol where you can discover and share optimizations with the research community.For use with λ DNA-Mono Cut Mix (N3019), фX174 DNA-HaeIII Digest (N3026),pBR322 DNA-BstNI Digest (N3031), pBR322 DNA-MspI Digest (N3032), 2-Log DNA Ladder (0.1-10.0 kb) (N3200), 100 bp DNA Ladder (N3231), 1 kb DNA Ladder (N3232), Low Molecular Weight DNA Ladder (N3233), and 50 bp DNA Ladder (N3236)
The following protocol is recommended for a 5 mm wide lane.
- Prepare loading mixture:
Distilled water - 4 μl
Gel Loading Dye, Purple (6X), no SDS - 1 μl
DNA Ladder - 1 μl
Total volume - 6 μl
- Mix gently
- Load onto the agarose gel
Note: The components of the mixture should be scaled up or down, depending on the width of the agarose gel.
For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH20.