Sample preparation using provided buffer and denaturing conditions (N0364)
This method utilizes the 2X ssRNA Ladder Loading Buffer provided and samples should be run on a denaturing polyacrylamide-urea gel prepared with 1X TBE.
- Combine on ice:
Low Range ssRNA Ladder (500 µg/ml): 0.5 µl (0.25 µg)
H2O (RNase-free): 4.5 µl
2X ssRNA Ladder Loading Buffer: 5 µl
Total Volume: 10 µl
- Heat at 65°C for 5 minutes, chill on ice, load entire sample on gel.