Reverse Transcription (E6160)

Protocol

1. Mix the following components in a sterile nuclease-free tube:
   3´ 5´ Ligated RNA from step 12: 11 μl
   Deoxynucleotide Solution Mix: 1 μl
   5X First Strand Buffer (Supplied with SuperScript® II): 4 μl
   0.1 M DTT (Supplied with SuperScript® II): 2 μl
   Murine RNase Inhibitor: 1 μl 
   ------------------------------------------------------------
   Total volume: 19 μl
2. Heat the sample for 2 minutes at 42°C.
3. Add 1 μl SuperScript® II Reverse Transcriptase.
4. Incubate for 1 hour at 42°C.